Rapid multiplexed analysis for molecular diagnostics
Patrick Doyle with Daniel Pregibon
![](https://deshpande.mit.edu/wp-content/uploads/2022/01/two_color_barcodes-aspect-ratio-580-360-580x360-c-default.jpg)
Continuous flow lithography
Molecular diagnostics is a multi-billion dollar market that directly impacts the progression in a variety of important medical research; from cancer to genomics. Despite the current use of microarrays in research labs; the technology is expensive, lacks versatility, has reproducibility issues, and is not well suited for applications in global medicine and point-of-care diagnostics. The development of a portable, cheap, simple multiplexing tool that can characterize DNA, RNA, and/or protein targets in a single patient sample would enable bedside diagnostics in clinical settings. This project will complete proof-of-principle experiments on this new method for multiplexed analysis using multifunctional microparticles created by continuous flow lithography. This method offers many advantages over existing technologies including: high multiplexing capabilities, simple 1-step particle fabrication, and versatility.
![](https://live-mit-deshpande.pantheonsite.io/wp-content/uploads/2022/01/Firefly20Optical20Liquid20Stampingmultiprobe.jpg)
Firefly BioWorks
The technology from this project was spun out into a startup company, Firefly BioWorks.