Continuous directed evolution in mammalian cells

Year 2021
Project team Matthew Shoulders & Amit Choudhary with Andrew Sabol and Amanuella Mengiste
Biomolecules have impacts from pharmaceuticals to biotech
Mammalian proteins difficult to direct through current methods
New system would enable rapid evolution of more proteins

New possibilities in biomedicine

Biomolecules engineered through directed evolution can have immense impacts, from pharmaceuticals to biotechnologies and beyond. Currently, directed evolution campaigns are performed in test tubes, bacteria or yeast. This approach can be tremendously problematic for most mammalian proteins or proteins intended to function in mammalian cells. This project strives to enable the rapid and continuous directed evolution of high-value protein targets, such as genome editors and molecular sensors, directly in mammalian cells. The system would allow researchers to target a wider range of proteins, opening new possibilities in biomedicine and biotechnology.

Andrew Sabol presents “Improving Directed Evolution in Mammalian Cells” at IdeaStream 2023
Samuel Hendel presents “Commercializing directed evolution in mammalian cells” at IdeaStream 2022